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Microarray
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Microarray
- Provides rapid and cost-effective methods of identifying gene
expression and genetic variation
- Gene expression profiling: allows one to screen large numbers of
genes to determine whether or not they are active under various
conditions
- Important for diagnosis and treatment of diseases
- Provides ability to study pathways related to specific functions
- Ability for quantitative analysis using ratios of expression levels
for individual genes
- Being used in a number of laboratory applications for a variety of
projects
Applications
 Gene expression analysis at the genomic level
- Polymorphism analysis
- Mapping
- Evolutionary studies
The Microarray Process
- Select clones, replicate, and perform PCR
- Coat slides w/ Poly-L-Lysine to adhere PCR product onto slide
- Stamp clones onto slides using an arrayer (right)
- RNA is extracted from tissue sample
- RNA is reverse transcribed to cDNA
- cDNA samples are tagged with fluorescent labels (ex: Cy3 and Cy5)
and are applied to the array
- Single stranded cDNA from sample bind (hybridize) to the
complementary strand of cDNA stamped on slide
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